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Bio soft sequencher download11/4/2022 ![]() ![]() ![]() Here we report a large scale survey on the distribution and genetic diversity of tomato infecting geminiviruses which are affecting mayor growing area of this crop in the country. However characterization of these new viruses had not been carried out so far. tabaci.ĭiseased caused by begomoviruses (Family Geminiviridae) constitute a serious constraint to tomato production in Colombia. En este estudio se discute la relación de los begomovirus con los graves perjuicios sufridos recientemente por los agricultores, debido a la baja en la producción de tomate, así como las estrategias para el manejo del complejo begomovirus-B. Estos resultados se confirmaron mediante secuenciación de ADN y análisis de secuencias. En las muestras analizadas no había presencia de TYMoV y ToLCSiV. El 100% de estas muestras resultó infectado con PYMPV. Cincuenta y ocho (58) muestras (82,9% del total) resultaron positivas a begomovirus. Las muestras con reacción positiva se analizaron con iniciadores específicos para las tres especies de begomovirus que infectan tomate en Panamá: virus del mosaico amarillo de la papa de Panamá (PYMPV), virus del moteado amarillo del tomate (TYMoV) y virus del enrollamiento de la hoja de tomate de Sinaloa (ToLCSiV). Se extrajo el ADN total de cada muestra y los extractos se analizaron mediante la técnica de reacción en cadena de la polimerasa (PCR), utilizando iniciadores degenerados para begomovirus. ![]() Se recolectaron 70 muestras de hojas en plantas de tomate con síntomas asociados a infecciones virales, en nueve parcelas de siete localidades, en las principales zonas productoras del cultivo. Con el objetivo de determinar la incidencia de begomovirus se realizó una prospección en el cultivo de tomate industrial en la provincia de Los Santos, Panamá, en el período 2016-2017. RESUMEN Los begomovirus, transmitidos por la mosca blanca Bemisia tabaci, constituyen el grupo de virus de mayor incidencia y distribución en el cultivo del tomate en los países tropicales y subtropicales. ![]() Finally, this paper reports by the first time to three alternative begomoviruses weeds hosts that infect tomato and passion fruit crops: A. dubius weeds also was found in this analysis. Mixed infections of begomoviruses in Desmodium sp. Cloned viruses fragments from these weeds could be new begomoviruses not previously reported before, this in accordance with the ICTV species demarcation criterion for the genus Begomovirus (≥91 % sequence identity). dubius, Rivina humilis, Desmodium sp., Rhynchosia minima, Hybanthus attenuatus, Verbena sp., Croton hirtus and Caesalpinia sp., showed its highest nucleotide sequence identity (89%) with bean chlorotic mosaic virus (BChMV), datura leaf distortion virus (DaLDV) and rhynchosia golden mosaic Yucatan virus (RhGMYV). and Amaranthus dubius were previously described as potato yellow mosaic virus (PYMV) and passionfruit leaf distortion virus (PLDV), respectively other four virus which were isolated from L. Nucleotide sequence analysis of viral fragments showed the presence of six different begomoviruses: two virus isolated from Lantana camara, Desmodium sp. For each sample, DNA fragments were cloned, sequenced and analyzed. These samples were positive for begomoviruses but their molecular characterization had not been done until now. Previously eleven samples of weeds were collected along the edges of a tomato crop field located in four municipalities (Florida, Ginebra, Cerrito and Candelaria) in the Southeast of Valle del Cauca. Weeds are usually considered as a source of new viruses but are often neglected during diversity studies. ![]()
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